Methods: Wash inclusion body effectivity, use Hydrophobic Interaction Chromatography to carry on the purification. 方法:对包涵体进行有效洗涤,采用疏水层析进行纯化。
Methods Purify plasmid DNA by a procedure consisting of alkaline lysis, ultrafiltration with hollow fiber filter, hydrophobic chromatography and molecular sieve chromatography. 方法采用碱裂解、中空纤维超滤浓缩、疏水层析、分子筛等分离技术纯化质粒DNA。
Studying the Retention Mechanism of Hydrophobic Interaction Chromatography by Using Aromatic Alcohol Homologues as Solute 用芳香醇同系物研究疏水色谱的保留机理
AIM: To investigate the refolding of the denatured proteins by high performance hydrophobic interaction chromatography ( HPHIC). 目的:研究变性蛋白质在高效疏水作用色谱(HPHIC)上的保留行为及复性效率。
A Study on Cholinesterase Immobilized by Agar with the Hydrophobic Groups Purification of rhIL-4 by Hydrophobic Chromatography 疏水载体固定化胆碱酯酶的研究疏水层析纯化人重组白细胞介素-4
Hydrophobic Membrane Chromatography for Fast Purification of Biological Macromolecules 疏水膜色谱法对生物大分子的快速纯化
The process study for the large scale purification of recombinant HBsAg by Hydrophobic Interaction Chromatography 疏水层析用于大规模纯化重组HBsAg的工艺研究
The basic principles and operation considerations of gel filtration chromatography, ion exchange chromatography and hydrophobic interaction chromatography were mainly clarified, and their applying strategies in separation and purification of protein were illustrated. 本文阐述了凝胶过滤、离子交换和疏水相互作用色谱的基本原理和操作条件,说明了它们在蛋白质纯化中的应用策略。
Denatured Thermodynamics for Proteins in Hydrophobic Interaction Chromatography 蛋白质在疏水色谱中的变性热力学
Large-scale Purification of Plasmid DNA by Ultrafiltration with Hollow Fiber Filter and Hydrophobic Chromatography 中空纤维超滤和疏水层析大规模纯化质粒DNA
Two different methods, direct coupling and coupling after activation, have been used to prepare the hydrophobic interaction chromatography media. 以交联琼脂糖为基质,采用直接偶联法和活化偶联法制备了苯基琼脂糖疏水层析介质。
Determination of Thermodynamic Parameters for Protein Retention in Hydrophobic Interaction Chromatography 蛋白质在疏水色谱中保留的热力学参数的测定
Advancement of Ion Exchange Chromatography and Hydrophobic Interaction Chromatography Media Application in Protein Chromatography 蛋白质层析用离子交换和疏水作用层析介质的发展概况
The Influence of Guanidine Hydrochloride on the Retention of Proteins in Hydrophobic Interaction Chromatography 盐酸胍对疏水色谱中蛋白质保留行为的影响
Study on Protein Conformational Changes in Hydrophobic Interaction Chromatography 使用疏水作用色谱研究蛋白质的构象变化
The retention behaviors of aromatic alcohol homologues in hydrophobic interaction chromatography ( HIC) were investigated firstly. 首次研究了芳香醇同系物在疏水色谱(HIC)中的保留行为。
Then, the solubilized rhG-CSF at alkaline solution was proceeded by protein folding liquid chromatography ( PFLC), including ion-exchange chromatography ( TEC), immobilized metal chelate affinity chromatography ( IMAC), hydrophobic interaction chromatography ( HIC), respectively. 然后用蛋白折叠液相色谱法(PFLC),包括阴离子交换色谱、金属离子亲和色谱和疏水色谱对rhG-CSF进行复性,提高了rhG-CSF在不同色谱模式下的质量回收率。
Prediction of Retention for Protein in Hydrophobic Interaction Chromatography 疏水相互作用色谱中蛋白质保留值的预测
Effect of composition of mobile phases on the retention behavior of solutes in hydrophobic interaction chromatography ( HIC) The retention behavior of aromatic alcohol homologues in the solutions of different salts in HIC was firstly investigated, and the retention behavior of proteins was also studied. 首次研究了疏水相互作用色谱(HIC)中芳香醇同系物在四种盐流动相中的保留行为,并对蛋白质在HIC中的保留行为进行了研究。
Reduced and denatured RNase A by using high performance hydrophobic interaction chromatography ( HPHIC) was studied. The effects of urea concentration, flow rate of mobile phase and protein concentration were also investigated. 用高效疏水色谱(HPHIC)研究了还原变性核糖核酸酶(RNaseA)在HIC上流动相中脲浓度、流动相流速及蛋白浓度对还原变性RNaseA复性的影响。
Hydrophobic interaction chromatography ( HIC) is an attractive method for protein purification. 疏水作用色谱法(HIC)是一种有效的蛋白质分离纯化方法。
Adsorption kinetics and equilibrium process of protein in hydrophobic interaction chromatography 疏水层析蛋白质动力学与平衡过程的考察
Synthesis of High Efficiency Packing and Using for Recombinant Human Interferons Separation in High-performance Hydrophobic Interaction Chromatography 高效疏水作用色谱填料的合成及其在重组人干扰素纯化中的应用
Hydrophobic interaction chromatography ( HIC), as one of important techniques in downstream bioprocessing, has been extensively applied in the purification of biological macromolecules and nanoparticles, such as proteins. 疏水性相互作用色谱(HIC)是一种有效的色谱分离手段,被广泛地应用于蛋白质等生物大分子和生物纳米颗粒的纯化中。
A new kind of chromatographic column called simple-type chromatographic column was designed and investigated by using the packings of hydrophobic interaction chromatography with large particles in this thesis. 新研制出一种简易型色谱柱并通过装填大颗粒的疏水色谱填料对色谱柱的性能进行了考察。